Western blot analysis of extracts of various cell lines, using OTUB1 antibody (A11656) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
OTUB1 Rabbit pAb
Catalog No:AB-A11656
Manufacturer:ABclonal
Synonyms:OTUB1;HSPC263;OTB1;OTU1
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | OTUB1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OTUB1 (NP_060140.2). |
Gene ID: | 55611 |
Swiss prot: | Q96FW1 |
Molecular weight: | 31kDa/35kDa |
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