Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 alpha antibody (A11526) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
PI3 Kinase p85 alpha Rabbit pAb
Catalog No:AB-A11526
Manufacturer:ABclonal
Synonyms:AGM7;GRB1;IMD36;p85;p85-ALPHA;PI3 Kinase p85 alpha;PIK3R1
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PI3 Kinase p85 alpha Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-380 of human PI3 Kinase p85 alpha (NP_852556.2). |
Gene ID: | 5295 |
Swiss prot: | P27986 |
Molecular weight: | 42kDa/49kDa/53kDa/83kDa/84kDa |
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