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Western blot analysis of extracts of various cell lines, using β-Catenin antibody (A11512) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Western blot analysis of extracts from normal (control) and β-Catenin knockout (KO) 293T cells, using β-Catenin antibody (A11512) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded rat pancreas using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse stomach using [KO Validated] β-Catenin Rabbit pAb (A11512) at dilution of 1:100 (40x lens).
[KO Validated] β-Catenin Rabbit pAb
Catalog No：AB-A11512
Manufacturer：ABclonal
Synonyms：CTNNB;MRD19;armadillo;beta Catenin;CTNNB1
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] β-Catenin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human β-Catenin (NP_001895.1). |
| Gene ID: | 1499 |
| Swiss prot: | P35222 |
| Molecular weight: | 9kDa/85kDa |
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