Western blot analysis of extracts of various cell lines, using ATG5 antibody (A11427) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
ATG5 Rabbit pAb
Catalog No:AB-A11427
Manufacturer:ABclonal
Synonyms:ATG5;APG5;APG5-LIKE;APG5L;ASP;hAPG5
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | ATG5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 28-275 of human ATG5 (NP_004840.1). |
Gene ID: | 9474 |
Swiss prot: | Q9H1Y0 |
Molecular weight: | 22kDa/32kDa |
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