.jpg)
Western blot analysis of extracts of various cell lines, using CD47 Rabbit mAb (A11382) at 1：1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
.jpg)
Western blot analysis of extracts of various cell lines, using CD47 Rabbit mAb (A11382) at 1：1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
.jpg)
Western blot analysis of extracts from normal (control) and CD47 knockout (KO) MCF7 cells and U2OS cells, using CD47 antibody (A11382) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
[KO Validated] CD47 Rabbit mAb
Catalog No：AB-A11382
Manufacturer：ABclonal
Synonyms：IAP, MER6, OA3
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | [KO Validated] CD47 Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010] |
| Immunogen: | A synthesized peptide derived from human CD47 |
| Gene ID: | 961 |
| Swiss prot: | Q08722 |
| Molecular weight: | 55kDa |
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