Western blot analysis of extracts of various cell lines, using MCM7 antibody (A1138) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
MCM7 Rabbit pAb
Catalog No:AB-A1138
Manufacturer:ABclonal
Synonyms:MCM7;CDC47;MCM2;P1.1-MCM3;P1CDC47;P85MCM;PNAS146;PPP1R104
Applications:WB,IF,IP
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | MCM7 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 360-719 of human MCM7 (NP_005907.3). |
Gene ID: | 4176 |
Swiss prot: | P33993 |
Molecular weight: | 44kDa/60kDa/81kDa |
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