Western blot analysis of extracts of C6 cells, using DDIT3 / CHOP antibody (A11346) at 1:1000 dilution.C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
DDIT3 / CHOP Rabbit pAb
Catalog No:AB-A11346
Manufacturer:ABclonal
Synonyms:DDIT3;CEBPZ;CHOP;CHOP-10;CHOP10;GADD153;C/EBPzeta;DDIT3 / CHOP
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DDIT3 / CHOP Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human DDIT3 / CHOP (NP_004074.2). |
Gene ID: | 1649 |
Swiss prot: | P35638 |
Molecular weight: | 19kDa/21kDa |
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