Western blot analysis of extracts of various cell lines, using Dot1L/KMT4 antibody (A11286) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
Dot1L/KMT4 Rabbit pAb
Catalog No:AB-A11286
Manufacturer:ABclonal
Synonyms:DOT1L;DOT1;KMT4
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Dot1L/KMT4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human Dot1L/KMT4 (NP_115871.1). |
Gene ID: | 84444 |
Swiss prot: | Q8TEK3 |
Molecular weight: | 164kDa/184kDa |
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