Western blot analysis of extracts of various cell lines, using KEAP1 antibody (A11258) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
KEAP1 Rabbit pAb
Catalog No:AB-A11258
Manufacturer:ABclonal
Synonyms:KEAP1;INrf2;KLHL19
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | KEAP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KEAP1 (NP_036421.2). |
Gene ID: | 9817 |
Swiss prot: | Q14145 |
Molecular weight: | 69kDa |
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