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Western blot analysis of extracts of various cell lines, using DAZL antibody (A1114) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
DAZL Rabbit pAb
Catalog No：AB-A1114
Manufacturer：ABclonal
Synonyms：DAZL;DAZH;DAZL1;DAZLA;SPGYLA
Applications：WB,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | DAZL Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 186-295 of human DAZL (NP_001342.2). |
| Gene ID: | 1618 |
| Swiss prot: | Q92904 |
| Molecular weight: | 33kDa/35kDa |
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