Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (A11097) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Histone H2AX Rabbit pAb
Catalog No:AB-A11097
Manufacturer:ABclonal
Synonyms:H2A.X;H2A/X;H2AX;Histone H2AX;H2AFX;histone H2AX;gamma H2A.X;γH2AX
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Histone H2AX Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human Histone H2AX (NP_002096.1). |
Gene ID: | 3014 |
Swiss prot: | P16104 |
Molecular weight: | 15kDa |
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