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Western blot analysis of extracts of HCT116 cells, using Bcl-2 antibody (A11025) at 1:1000 dilution.HCT116 cells treated with different concentrations of B7-H4.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
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Western blot analysis of extracts of various cell lines, using Bcl-2 antibody (A11025) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human thyroid cancer using Bcl-2 Rabbit pAb (A11025) at dilution of 1:100 (40x lens).
Bcl-2 Rabbit pAb
Catalog No：AB-A11025
Manufacturer：ABclonal
Synonyms：Bcl-2;PPP1R50;BCL2
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Bcl-2 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2). |
| Gene ID: | 596 |
| Swiss prot: | P10415 |
| Molecular weight: | 22kDa/26kDa |
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