Western blot analysis of extracts of various cell lines, using Phospho-CGAS-S420 antibody (AP1228) at 1:1000 dilution.293T and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-CGAS-S420 Rabbit pAb
Catalog No:AB-AP1228
Manufacturer:ABclonal
Synonyms:E330016A19Rik, cGAS
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€150,00 – €410,00
Product Name: | Phospho-CGAS-S420 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a DNA binding cytosolic protein that catalyzes the synthesis of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) after sensing the presence of DNA in the cytoplasm. cGAMP binds another protein, Stimulator of interferon genes (STING), leading to the induction of interferons, and a host immune response. Reduced expression of this gene inhibits interferon induction in the presence of some viral infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Immunogen: | A phospho synthetic peptide corresponding to residues surrounding S420 of Mouse CGAS. |
Gene ID: | 214763 |
Swiss prot: | Q8C6L5 |
Molecular weight: |
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