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Western blot analysis of extracts of HeLa cells, using Phospho-NF2-S518 antibody (AP1225) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-NF2-S518 Rabbit pAb
Catalog No：AB-AP1225
Manufacturer：ABclonal
Synonyms：NF2;ACN;BANF;SCH;merlin
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-NF2-S518 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. |
| Immunogen: | A synthetic phosphorylated peptide around S518 of human NF2 (NP_000259.1). |
| Gene ID: | 4771 |
| Swiss prot: | P35240 |
| Molecular weight: | 19-30kDa/59-72kDa |
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