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Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1173 antibody (AP1213) at 1:500 dilution.A-431 cells were treated by EGF (25 ug/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-EGFR-Y1173 Rabbit pAb
Catalog No：AB-AP1213
Manufacturer：ABclonal
Synonyms：ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-EGFR-Y1173 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y1173 of human EGFR |
| Gene ID: | 1956 |
| Swiss prot: | P00533 |
| Molecular weight: | 175kDa |
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