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Western blot analysis of extracts of various cell lines, using Phospho-LAT-Y191 antibody (AP1188) at 1:1000 dilution.Jurkat cells were treated by CD-3 (10 ug/mL) at 37℃ for 2 minutes after serum-starvation overnight.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-LAT-Y191 Rabbit pAb
Catalog No：AB-AP1188
Manufacturer：ABclonal
Synonyms：LAT;LAT1;pp36;IMD52
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-LAT-Y191 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y191 of human LAT |
| Gene ID: | 27040 |
| Swiss prot: | O43561 |
| Molecular weight: | 24kDa/27kDa/28kDa |
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