Western blot analysis of extracts of A-431 cells, using Phospho-SHC1-Y427 antibody (AP1184) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-SHC1-Y427 Rabbit pAb
Catalog No:AB-AP1184
Manufacturer:ABclonal
Synonyms:SHC;SHCA;SHC1
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-SHC1-Y427 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y427 of human SHC1. |
Gene ID: | 6464 |
Swiss prot: | P29353 |
Molecular weight: |
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