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Western blot analysis of extracts of HeLa cells, using Phospho-p53-S367 antibody (AP1170) at 1:500 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-p53-S367 Rabbit pAb
Catalog No：AB-AP1170
Manufacturer：ABclonal
Synonyms：TP53; BCC7; LFS1; P53; TRP53; tumor protein p53
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-p53-S367 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S367 of human TP53. |
| Gene ID: | 7157 |
| Swiss prot: | P04637 |
| Molecular weight: | 23-43kDa |
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