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Western blot analysis of extracts of various cell lines, using Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (AP1158) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb
Catalog No：AB-AP1158
Manufacturer：ABclonal
Synonyms：PAKalpha
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S144 of human PAK1 |
| Gene ID: | 5058/5062/5063 |
| Swiss prot: | Q13153/Q13177/O75914 |
| Molecular weight: | 65kDa |
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