Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Cdc6-S54 Rabbit mAb (AP1153) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (1 uM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-Cdc6-S54 Rabbit mAb
Catalog No:AB-AP1153
Manufacturer:ABclonal
Synonyms:CDC18L, HsCDC18, HsCDC6, MGORS5
Applications:WB
Reactivity:Human,Mouse,
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-Cdc6-S54 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S54 of human Cdc6 |
Gene ID: | 990 |
Swiss prot: | Q99741 |
Molecular weight: | 63kDa |
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