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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H3.3-T3 Rabbit mAb (AP1152) at 1:1000 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
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Western blot analysis of extracts of HeLa cells, using Phospho-Histone H3.3-T3 Rabbit mAb (AP1152) at 1:1000 dilution.HeLa cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of C6 cells, using Phospho-Histone H3.3-T3 Rabbit mAb (AP1152) at 1:1000 dilution.C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
Phospho-Histone H3.3-T3 Rabbit mAb
Catalog No：AB-AP1152
Manufacturer：ABclonal
Synonyms：H3.3A, H3F3
Applications：WB
Reactivity：Human,Mouse,Rat,Other (Wide Range)
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Histone H3.3-T3 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T3 of human Histone H3 |
| Gene ID: | 3020 |
| Swiss prot: | P84243 |
| Molecular weight: | 16kDa |
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