Western blot analysis of extracts of various cell lines, using Phospho-CDC37-S13 Rabbit mAb (AP1141) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of C6 cells, using Phospho-CDC37-S13 Rabbit mAb (AP1141) at 1:1000 dilution.C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-CDC37-S13 Rabbit mAb
Catalog No:AB-AP1141
Manufacturer:ABclonal
Synonyms:P50CDC37
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-CDC37-S13 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S13 of human Phospho-CDC37-S13 |
Gene ID: | 11140 |
Swiss prot: | Q16543 |
Molecular weight: | 50kDa |
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