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Western blot analysis of extracts of 293T cells, using Phospho-HSF1-S326 Rabbit mAb (AP1140) at 1:1000 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
Phospho-HSF1-S326 Rabbit mAb
Catalog No：AB-AP1140
Manufacturer：ABclonal
Synonyms：HSTF1
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-HSF1-S326 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S326 of human HSF1 |
| Gene ID: | 3297 |
| Swiss prot: | Q00613 |
| Molecular weight: | 82kDa |
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