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Western blot analysis of extracts of various cell lines, using Phospho-CDK1/2/3-T14 Rabbit mAb (AP1138) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours or treated by Nocodazole (100 ng/mL) at 37℃ for 24 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-CDK1/2/3-T14 Rabbit mAb
Catalog No：AB-AP1138
Manufacturer：ABclonal
Synonyms：CDC2, CDC28A, P34CDC2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-CDK1/2/3-T14 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T14 of human CDK1/2/3 |
| Gene ID: | 983/1017/1018 |
| Swiss prot: | P06493/P24941/Q00526 |
| Molecular weight: | 34kDa |
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