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Western blot analysis of extracts of various cell lines, using Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb (A4826) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb
Catalog No：AB-AP1132
Manufacturer：ABclonal
Synonyms：H1.3, H1D, H1F3, H1s-2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Histone H1.3-T17/Histone H1.4-T17 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T17 of human Histone H1.3 |
| Gene ID: | 3007/3008 |
| Swiss prot: | P16402/P10412 |
| Molecular weight: | 30kDa |
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