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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Western blot analysis of extracts of C6 cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts of HeLa cells, using Phospho-Vimentin-S83 antibody (AP1119) at 1:1000 dilution.HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours.HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-Vimentin-S83 Rabbit pAb
Catalog No：AB-AP1119
Manufacturer：ABclonal
Synonyms：VIM; CTRCT30; HEL113; vimentin
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Vimentin-S83 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S83 of human VIM. |
| Gene ID: | 7431 |
| Swiss prot: | P08670 |
| Molecular weight: | 53kDa |
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