Western blot analysis of extracts of 293T cells, using Phospho-Ezrin-Y353 pAb (AP1118) at 1:1000 dilution or Ezrin antibody (A0703).293T cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-Ezrin-Y353 Rabbit pAb
Catalog No:AB-AP1118
Manufacturer:ABclonal
Synonyms:EZR; CVIL; CVL; HEL-S-105; VIL2; ezrin
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Ezrin-Y353 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y353 of human Ezrin. |
Gene ID: | 7430 |
Swiss prot: | P15311 |
Molecular weight: | 69kDa |
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