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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EGFR-T678 antibody (AP1114) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
Phospho-EGFR-T678 Rabbit pAb
Catalog No：AB-AP1114
Manufacturer：ABclonal
Synonyms：EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA; epidermal growth factor receptor
Applications：WB
Reactivity：Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-EGFR-T678 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T678 of human EGFR. |
| Gene ID: | 1956 |
| Swiss prot: | P00533 |
| Molecular weight: | 44kDa/69kDa/77kDa/134kDa |
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