Western blot analysis of extracts of PC-12 cells, using Phospho-Tyrosine Hydroxylase-S40 pAb (AP1109) at 1:1000 dilution or Tyrosine Hydroxylase antibody (A0028).PC-12 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Phospho-Tyrosine Hydroxylase-S40 Rabbit pAb
Catalog No:AB-AP1109
Manufacturer:ABclonal
Synonyms:DYT14;DYT5b;TYH;TH
Applications:WB
Reactivity:Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Tyrosine Hydroxylase-S40 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S40 of human Tyrosine Hydroxylase. |
Gene ID: | 7054 |
Swiss prot: | P07101 |
Molecular weight: | 44kDa/45kDa/55kDa/56kDa/58kDa |
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