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Western blot analysis of extracts of HeLa cells, using Phospho-E-Cadherin-S838/S840 antibody (AP1074) at 1:1000 dilution.HeLa cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-E-Cadherin-S838/S840 Rabbit pAb
Catalog No：AB-AP1074
Manufacturer：ABclonal
Synonyms：Arc-1;CD324;CDHE;ECAD;LCAM;UVO;CDH1;E-Cadherin
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-E-Cadherin-S838/S840 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015] |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding S838/S840 of human CDH1. |
| Gene ID: | 999 |
| Swiss prot: | P12830 |
| Molecular weight: | 90kDa/97kDa |
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