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Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 antibody (AP1068) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat lung using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb (AP1068) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb (AP1068) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb (AP1068) at dilution of 1:100 (40x lens).
Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb
Catalog No：AB-AP1068
Manufacturer：ABclonal
Synonyms：AKT1/AKT2/AKT3
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011] |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding S473/S474/S472 of human AKT1/AKT2/AKT3. |
| Gene ID: | 207/208/10000 |
| Swiss prot: | P31749/P31751/Q9Y243 |
| Molecular weight: | 48kDa/55kDa/51kDa/54kDa |
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