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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Cyclin D1-T286 pAb (AP1061) at 1:1000 dilution or Cyclin D1 antibody (A11310).NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat eye using Phospho-Cyclin D1-T286 Rabbit pAb (AP1061) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-Cyclin D1-T286 Rabbit pAb (AP1061) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse eye using Phospho-Cyclin D1-T286 Rabbit pAb (AP1061) at dilution of 1:100 (40x lens).
Phospho-Cyclin D1-T286 Rabbit pAb
Catalog No：AB-AP1061
Manufacturer：ABclonal
Synonyms：BCL1;D11S287E;PRAD1;U21B31;CCND1;Cyclin D1;cyclin D1
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Cyclin D1-T286 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding T286 of human Cyclin D1. |
| Gene ID: | 595 |
| Swiss prot: | P24385 |
| Molecular weight: | 33kDa |
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