Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (AP1051) at 1:1000 dilution.NIH/3T3 cells were treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Phospho-ATF2-T71 Rabbit mAb
Catalog No:AB-AP1051
Manufacturer:ABclonal
Synonyms:CRE-BP1, CREB-2, CREB2, HB16, TREB7
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-ATF2-T71 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T71 of human ATF2 |
Gene ID: | 1386 |
Swiss prot: | P15336 |
Molecular weight: | 70kDa |
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