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Western blot analysis of extracts of various cell lines, using Phospho-TBK1/NAK-S172 Rabbit mAb (AP1026) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.
Phospho-TBK1/NAK-S172 Rabbit mAb
Catalog No：AB-AP1026
Manufacturer：ABclonal
Synonyms：FTDALS4, NAK, T2K
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-TBK1/NAK-S172 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S172 of human TBK1/NAK |
| Gene ID: | 29110 |
| Swiss prot: | Q9UHD2 |
| Molecular weight: | 84kDa |
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