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Western blot analysis of extracts of various cell lines, using Phospho-PDHA1-S293 Rabbit mAb (AP1022) at 1:1000 dilution.Hela cells and NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Phospho-PDHA1-S293 Rabbit mAb
Catalog No：AB-AP1022
Manufacturer：ABclonal
Synonyms：PDHA, PDHAD, PDHCE1A, PHE1A
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-PDHA1-S293 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S293 of human PDHA1 |
| Gene ID: | 5160 |
| Swiss prot: | P08559 |
| Molecular weight: | 40kDa |
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