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Western blot analysis of extracts of A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb (AP1018) at 1:1000 dilution.A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-Rac1/Cdc42-S71 Rabbit mAb
Catalog No：AB-AP1018
Manufacturer：ABclonal
Synonyms：CDC42Hs, G25K, TKS
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Rac1/Cdc42-S71 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S71 of human Rac1/Cdc42 |
| Gene ID: | 998/5879 |
| Swiss prot: | P60953/P63000 |
| Molecular weight: | 24kDa |
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