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Western blot analysis of extracts of various cell lines, using Phospho-Raf1-S259 Rabbit mAb (AP1012) at 1:1000 dilution.Both 293T cells and C6 cells were treated by Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-Raf1-S259 Rabbit mAb
Catalog No：AB-AP1012
Manufacturer：ABclonal
Synonyms：CMD1NN, CRAF, NS5, Raf-1, c-Raf
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Raf1-S259 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S259 of human Phospho-Raf1-S259 |
| Gene ID: | 5894 |
| Swiss prot: | P04049 |
| Molecular weight: | 74kDa |
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