Western blot analysis of extracts of HeLa cells, using Phospho-IRF3-S386 Rabbit mAb (AP0995) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-IRF3-S386 Rabbit mAb
Catalog No:AB-AP0995
Manufacturer:ABclonal
Synonyms:IIAE7
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-IRF3-S386 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S386 of human IRF3 |
Gene ID: | 3661 |
Swiss prot: | Q14653 |
Molecular weight: | 47kDa |
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