Western blot analysis of extracts of BxPC-3 cells, using Phospho-EGFR-Y1173 Rabbit mAb (AP0992) at 1:1000 dilution.BxPC-3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-EGFR-Y1173 Rabbit mAb
Catalog No:AB-AP0992
Manufacturer:ABclonal
Synonyms:ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-EGFR-Y1173 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y1173 of human EGFR |
Gene ID: | 1956 |
Swiss prot: | P00533 |
Molecular weight: | 175kDa |
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