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Western blot analysis of extracts of 293T cells, using Phospho-SIRT1-S47 antibody (AP0976) at 1:500 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-SIRT1-S47 Rabbit mAb
Catalog No：AB-AP0976
Manufacturer：ABclonal
Synonyms：SIR2, SIR2L1, SIR2alpha
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-SIRT1-S47 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S47 of human SIRT1 |
| Gene ID: | 23411 |
| Swiss prot: | Q96EB6 |
| Molecular weight: | 82kDa |
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