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Western blot analysis of extracts of MCF7 cells, using Phospho-POLR2A-S7 antibody (AP0969) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37℃ for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-POLR2A-S7 Rabbit pAb
Catalog No：AB-AP0969
Manufacturer：ABclonal
Synonyms：POLR2A;POLR2;POLRA;RPB1;RPBh1;RPO2;RPOL2;RpIILS;hRPB220;hsRPB1;Pol II
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €425,00
| Product Name: | Phospho-POLR2A-S7 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. |
| Immunogen: | A synthetic phosphorylated peptide around of human Phospho-POLR2A-S7. |
| Gene ID: | 5430 |
| Swiss prot: | P24928 |
| Molecular weight: | 63kDa/217kDa |
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