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Western blot analysis of extracts of various cell lines, using Phospho-PLN-S16/T17 antibody (AP0956) at 1:1000 dilution.Hela cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-PLN-S16/T17 Rabbit pAb
Catalog No：AB-AP0956
Manufacturer：ABclonal
Synonyms：CMD1P;CMH18;PLB;PLN
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €425,00
| Product Name: | Phospho-PLN-S16/T17 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. |
| Immunogen: | A synthetic phosphorylated peptide around of human Phospho-PLN-S16/T17. |
| Gene ID: | 5350 |
| Swiss prot: | P26678 |
| Molecular weight: | 6kDa |
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