Western blot analysis of extracts of various cell lines, using Phospho-MYL9-T18/S19 antibody (AP0955) at 1:1000 dilution.293T cells and C2C12 and C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-MYL9-T18/S19 Rabbit pAb
Catalog No:AB-AP0955
Manufacturer:ABclonal
Synonyms:MYL9;LC20;MLC-2C;MLC2;MRLC1;MYRL2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €425,00
Product Name: | Phospho-MYL9-T18/S19 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | A synthetic phosphorylated peptide around of human Phospho-MYL9-T18/S19 . |
Gene ID: | 10398 |
Swiss prot: | P24844 |
Molecular weight: | 13kDa/19kDa |
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