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Western blot analysis of extracts of 293T cells, using Phospho-MCL1-S159 antibody (AP0947) at 1:1000 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.293T cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-MCL1-S159 Rabbit pAb
Catalog No：AB-AP0947
Manufacturer：ABclonal
Synonyms：MCL1;BCL2L3;EAT;MCL1-ES;MCL1L;MCL1S;Mcl-1;TM;bcl2-L-3;mcl1/EAT;MCL1
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-MCL1-S159 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. |
| Immunogen: | A synthetic phosphorylated peptide around S159 of human MCL1. |
| Gene ID: | 4170 |
| Swiss prot: | Q07820 |
| Molecular weight: | 37kDa |
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