.jpg)
Western blot analysis of extracts of various cell lines, using Phospho-NF-kB p65/RelA-S529 antibody (AP0944) at 1:1000 dilution.HeLa cells were treated by both TNF-ɑ(20ng/mL) and Calyculin A (100nM) for 10 minutes.NIH/3T3 cells were treated by TNF-ɑ(20ng/mL) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
.jpg)
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-NF-kB p65/RelA-S529 pAb (AP0944) at 1:1000 dilution or NF-kB p65 antibody (A16271).NIH/3T3 cells were treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
.jpg)
Immunohistochemistry of paraffin-embedded rat lung using Phospho-NF-kB p65/RelA-S529 Rabbit pAb (AP0944) at dilution of 1:100 (40x lens).
Phospho-NF-kB p65/RelA-S529 Rabbit pAb
Catalog No：AB-AP0944
Manufacturer：ABclonal
Synonyms：NFKB3;p65;NF-kB p65;RELA;CMCU
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-NF-kB p65/RelA-S529 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S529 of human Phospho-NF-kB p65/RelA-S529. |
| Gene ID: | 5970 |
| Swiss prot: | Q04206 |
| Molecular weight: | 60kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No：AB-AP0027
Manufacturer：ABclonal
Synonyms：EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
Catalog No：AB-AP0025
Manufacturer：ABclonal
Synonyms：EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications：WB,IP
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0021
Manufacturer：ABclonal
Synonyms：ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0016
Manufacturer：ABclonal
Synonyms：CDK1;CDC2;CDC28A;P34CDC2
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0015
Manufacturer：ABclonal
Synonyms：CDK1;CDC2;CDC28A;P34CDC2
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0013
Manufacturer：ABclonal
Synonyms：CCNE1;CCNE;pCCNE1;cyclin E1
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00