Western blot analysis of extracts of various cell lines, using Phospho-(Ser/Thr) ATM/ATR Substrate pAb (AP0933) at 1:1000 dilution.Jurkat and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb
Catalog No:AB-AP0933
Manufacturer:ABclonal
Applications:WB
Reactivity:ALL
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-(Ser/Thr) ATM/ATR Substrate Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Ser/Thr of ATM/ATR Substrate. |
Gene ID: | |
Swiss prot: | |
Molecular weight: |
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