Western blot analysis of extracts of various cells, using Phospho-PPP2CA/PPP2CB-Y307 antibody (AP0927) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb
Catalog No:AB-AP0927
Manufacturer:ABclonal
Synonyms:PPP2CA/PPP2CB
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding Y307 of human PPP2CA/PPP2CB |
Gene ID: | 5515/5516 |
Swiss prot: | P67775/P62714 |
Molecular weight: |
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