Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0913) at 1:1000 dilution or ATF2 antibody (A2155).293T cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-ATF2-T69/71 Rabbit pAb
Catalog No:AB-AP0913
Manufacturer:ABclonal
Synonyms:CRE-BP1;CREB-2;CREB2;HB16;TREB7;ATF2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-ATF2-T69/71 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T69/71 of human ATF2. |
Gene ID: | 1386 |
Swiss prot: | P15336 |
Molecular weight: | 13- 15kDa/23-24kDa/35-54kDa |
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