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Western blot analysis of extracts of HeLa cells, using phospho-LATS1-T1079/LATS2-T1041 antibody (AP0912) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
phospho-LATS1-T1079/LATS2-T1041 Rabbit pAb
Catalog No：AB-AP0912
Manufacturer：ABclonal
Synonyms：LATS1;WARTS;wts
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | phospho-LATS1-T1079/LATS2-T1041 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T1079 of human LATS1. |
| Gene ID: | 9113/26524 |
| Swiss prot: | O95835/Q9NRM7 |
| Molecular weight: | 76kDa/126kDa |
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