Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T6 antibody (AP0899) at 1:1000 dilution.Hela cells and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 1s.
Phospho-Histone H3-T6 Rabbit pAb
Catalog No:AB-AP0899
Manufacturer:ABclonal
Synonyms:HIST1H3A; H3/A; H3FA; histone H3.1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat,Other (Wide Range)
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-Histone H3-T6 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
Immunogen: | |
Gene ID: | 8350 |
Swiss prot: | P68431 |
Molecular weight: | 15kDa |
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